S80331 |
JNK-IN-8 |
源葉(MedMol) | 99% |
- 提示:詳情請(qǐng)下載說明書。
- 產(chǎn)品描述: JNK-IN-8 is an irreversible JNK1/2/4 inhibitor (IC50: 4.7/18.7/1 nM). The selectivity is higher 10-fold than MNK2, Fms and no inhibition of Met, c-Kit, PDGFRβ in A375 cell line.
- 靶點(diǎn): c-Kit JNK;JNK;c-Kit
- 體內(nèi)研究:
JNK-IN-8(10 mM)對(duì)IL-1R細(xì)胞中IL-1β刺激的c-Jun磷酸化有抑制作用。與伊馬替尼相比,JNK-IN-8有明顯的1,4-雙苯胺和1,3-氨基苯甲酸結(jié)構(gòu)區(qū)域選擇性,且以N,N-二甲基丁烯乙酰胺共價(jià)結(jié)合Cys154靶點(diǎn)。JNK-IN-8抑制HeLa(EC50:486 nM)和A375(EC50:338 nM)細(xì)胞中c-Jun的磷酸化。JNK-IN-8與PIK3C3,IRAK1,PIP5K3和PIP4K2C結(jié)合可使選擇性和消除率顯著提高。JNK-IN-8經(jīng)Cys116抑制JNK2
- 細(xì)胞實(shí)驗(yàn): JNK-IN-8 is dissolved in DMSO and stored, and then diluted with appropriate media before use. HEK-293 cells stably expressing Interleukin Receptor 1 (HEK293-IL1R) are cultured in Dulbecco's Modified Eagle's medium (DMEM) supplemented with 10% FBS, 2 mM glutamine and 1×antimycotic/antibiotic solution. Cells are serum starved for 18 h before incubation with DMSO or JNK-IN-8, stimulated with 2 μM Anisomycin for 1h and lysates are clarified by centrifugation for 10 min at 16000 g and 4°C
- 參考文獻(xiàn):
1.Liu Q, et al. Chem Biol, 2013, 20(2), 146-159. 2.Zhang T, et al. Chem Biol, 2012, 19(1), 140-154. 3.Goh ET, et al. Biochem J, 2012, 441(1), 339-346.
- 溶解性: Ethanol:<1 mg/mL DMSO:93 mg/mL (183.2 mM) H2O:<1 mg/mL
- 保存條件: -20℃
- 配置溶液濃度參考:
1mg 5mg 10mg 1 mM 1.97 ml 9.85 ml 19.701 ml 5 mM 0.394 ml 1.97 ml 3.94 ml 10 mM 0.197 ml 0.985 ml 1.97 ml 50 mM 0.039 ml 0.197 ml 0.394 ml
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輸入產(chǎn)品批號(hào):
本計(jì)算器可幫助您計(jì)算出特定溶液中溶質(zhì)的質(zhì)量、溶液濃度和體積之間的關(guān)系,公式為:
質(zhì)量 (mg) = 濃度 (mM) x 體積 (mL) x 分子摩爾量 (g/mol)