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• 產(chǎn)品描述： JNK-IN-8 is an irreversible JNK1/2/4 inhibitor (IC50: 4.7/18.7/1 nM). The selectivity is higher 10-fold than MNK2, Fms and no inhibition of Met, c-Kit, PDGFRβ in A375 cell line.

• 靶點(diǎn)： c-Kit JNK;JNK;c-Kit

• 體內(nèi)研究：
  JNK-IN-8（10 mM）對(duì)IL-1R細(xì)胞中IL-1β刺激的c-Jun磷酸化有抑制作用。與伊馬替尼相比,JNK-IN-8有明顯的1,4-雙苯胺和1,3-氨基苯甲酸結(jié)構(gòu)區(qū)域選擇性,且以N,N-二甲基丁烯乙酰胺共價(jià)結(jié)合Cys154靶點(diǎn)。JNK-IN-8抑制HeLa（EC50：486 nM）和A375（EC50：338 nM）細(xì)胞中c-Jun的磷酸化。JNK-IN-8與PIK3C3,IRAK1,PIP5K3和PIP4K2C結(jié)合可使選擇性和消除率顯著提高。JNK-IN-8經(jīng)Cys116抑制JNK2

• 細(xì)胞實(shí)驗(yàn)： JNK-IN-8 is dissolved in DMSO and stored, and then diluted with appropriate media before use. HEK-293 cells stably expressing Interleukin Receptor 1 (HEK293-IL1R) are cultured in Dulbecco's Modified Eagle's medium (DMEM) supplemented with 10% FBS, 2 mM glutamine and 1×antimycotic/antibiotic solution. Cells are serum starved for 18 h before incubation with DMSO or JNK-IN-8, stimulated with 2 μM Anisomycin for 1h and lysates are clarified by centrifugation for 10 min at 16000 g and 4°C

• 參考文獻(xiàn)：
  1.Liu Q, et al. Chem Biol, 2013, 20(2), 146-159. 2.Zhang T, et al. Chem Biol, 2012, 19(1), 140-154. 3.Goh ET, et al. Biochem J, 2012, 441(1), 339-346.

• 溶解性： Ethanol:<1  mg/mL    DMSO:93  mg/mL  (183.2  mM)    H2O:<1  mg/mL

• 保存條件： -20℃

• 配置溶液濃度參考：
  

  	1mg	5mg	10mg
  1 mM	1.97 ml	9.85 ml	19.701 ml
  5 mM	0.394 ml	1.97 ml	3.94 ml
  10 mM	0.197 ml	0.985 ml	1.97 ml
  50 mM	0.039 ml	0.197 ml	0.394 ml

• 注意：部分產(chǎn)品我司僅能提供部分信息，我司不保證所提供信息的權(quán)威性，僅供客戶參考交流研究之用。

• 1. [IF=7.5] Jiashe Chen et al."Chlorogenic acid attenuates deoxynivalenol-induced apoptosis and pyroptosis in human keratinocytes via activating Nrf2/HO-1 and inhibiting MAPK/NF-κB/NLRP3 pathways."BIOMEDICINE & PHARMACOTHERAPY.2024 Jan;170:116003

輸入產(chǎn)品批號(hào)：

本計(jì)算器可幫助您計(jì)算出特定溶液中溶質(zhì)的質(zhì)量、溶液濃度和體積之間的關(guān)系，公式為：

質(zhì)量 (mg) = 濃度 (mM) x 體積 (mL) x 分子摩爾量 (g/mol)

• pg ng μg mg g kg =
  fM pM nM μM mM M *
  nL μL mL L *